DNA

Part:BBa_K4897000:Design

Designed by: Ji Zhiyuan   Group: iGEM23_BS-United-China   (2023-10-03)


BS DNA-50 (BS DNA 1.0) using in L-RCA for detecting P. acne


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 27
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 27
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 27
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Normal Taq enzyme may not appropriate in this DNA rolling cycle amplification chain reaction.


Source

the binding region is specific binding to P. acne 16S rRNA gene

References